MANNA - project 02
02it es

Nutrition and immunity: molecular approaches

Autonomous University of Barcelona
http://www.uab.cat/
Dr. Muriel Bonnet
INRA (France)
http://www.ara.inra.fr/
MetLabs (Slovak Republic)
http://metlabs.sk/

TAGS: In vitro studies, White Blood cells, avian and mammalian innate immunity;

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Objectives

The aim of the project is to define the in vitro impact of molecules used in nutrition studies of other MANNA ESR studies.

Description of the project

The Early Stage Researcher (ESR) involves the characterization of the in vitro effects of M. cordata extract, exopolysaccharides (EPS), exosomes, polyunsaturated fatty acids (PUFA) and microRNA, in avian and mammalian immune defence. The ESR will apply (or develop where not available) protocols to purify and culture immune related cells in pigs, ruminants and chicken, that will be co-incubated with the molecules utilized by other ESR within the MANNA project (FA, carotenoids and M. cordata extracts, EPS, purified milk exosomes and xenoMiR present in milk exosomes. Main cell activities will be measured, including chemotaxis, degranulation, ROS production, phagocytosis for phagocytes, and cell proliferation and MHC presentation of lymphocytes. The secretome from cultures will be analysed by proteomics and microRNAomics. Expression of immune related microRNA and genes will be carried out by means of open array™ or other technologies.

The ESR candidate student will carry out her/his PhD thesis study work in the Department of Veterinary Medicine at the University of Milan (http://www.unimi.it/) under the supervision of Prof. Fabrizio Ceciliani and the Autonomous University of Barcelona (http://www.uab.cat/), under the supervision of Prof. Armand Sanchez Bonastre. The supervision team is completed by Dr Muriel Bonnet (http://www.researcherid.com/rid/M-6265-2017), at INRA (http://www.ara.inra.fr/) in the Herbivores Unit (http://umrh-bioinfo.clermont.inra.fr/Intranet/web/UMRH/en).

The research will also involves short secondments at INRA (http://www.ara.inra.fr/) in Clermont Ferrand, France, and MetLabs (http://metlabs.sk/) at Bratislava, Slovakia.

The selected candidate will participate in the network’s training activities and work placements in the laboratories of the participating academic and industrial partners.