MANNA - project 04
04gb sk

Protein synthesis rates, effect of diet, health and innate immune responses in chicken

University of Veterinary Medicine and Pharmacy in Kosice
http://www.uvlf.sk/en
University of Zagreb
http://www.unizg.hr/homepage/
Dr. Chris Chadwick
Life Diagnostics (UK)
https://lifediagnostics.com/

MetLabs (Slovak Republic)
http://metlabs.sk/

TAGS: Fractional Synthesis Rate; deuterium oxide; chickens;

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Objectives

The aim of the project is to determine the rate of protein synthesis in tissues of chicken following endotoxin challenge and the effect of diet on this response.

Description of the project

The Early Stage Researcher (ESR) will study the fractional synthesis rate (FSR) of multiple target proteins in tissues from broiler chickens and assess the effects of using natural immunomodulatory compounds as supplements in their diet to the FSR under challenge by bacterial endotoxin. The ESR will focus on determining the FSR of selected target proteins by supplementing the diets with deuterium oxide (2H2O) and determining FSR by gas chromatography and mass spectrometry. The analytical spectrum will then be extended to OMIC analysis on selective proteins such as ovotransferrin, myosin, albumin and other plasma proteins with their relative synthesis rates determined during endotoxin challenge.

The ESR will be enrolled in the University of Glasgow (https://www.gla.ac.uk/) under the supervision of Professor Tom Preston, Scottish Universities Environmental Research Centre, and will be awarded a Double Doctorate degree in co-tutelle with the University of Veterinary Medicine and Pharmacy in Kosice (http://www.uvlf.sk/en), under the supervision of Professor Mangesh Bhide. The supervision team is completed by a non academic supervisor, Dr. Chris Chadwick, of Life Diagnostics (https://lifediagnostics.com/), UK.

The research will involve secondments to the University of Zagreb (http://www.unizg.hr/homepage/), Croatia, AB Agri (https://www.abagri.com/home) UK and MetLabs (http://metlabs.sk/), Slovak Republic.

The selected candidate will participate in the network’s training activities and work placements in the laboratories of the participating academic and industrial partners.