TAGS: 16S Sequencing; pigs; Deep-Sequencing techniques;
To characterize the microbiome (bacterial, fungal and archaeal) of porcine gut and faecal samples using Deep-sequencing techniques.
Description of the project
The project linked to the Early Stage Researcher (ESR) will characterize the microbiome (bacterial, fungal and archaeal) of porcine gut and faecal samples using Deep-sequencing techniques. The ESR will receive faecal and intestinal samples from pig reared in different conditions coming from studies done by ESR working on other MANNA projects and from additional pigs (2 different breeds) to compare 16S sequencing and deep sequencing for their informative value about the entire gut microbiome and to characterize the microbiome under the different feeding conditions applied in the pig experiments. Experiments on relatedness of gut and faecal samples profiles of the porcine microbiome will also be carried out. Besides metabolomics and proteomics will be applied and data will be evaluated to identify biomarkers for innovative breeding strategies.
The ESR will be enrolled by the Autonomous University of Barcelona (http://www.uab.cat/), Spain, under the supervision of Prof. Armand Sanchez Bonastre and will be awarded a Double Doctorate degree in co-tutelle with the University of Glasgow (https://www.gla.ac.uk/), UK, under the supervision of Dr Mark McLaughlin. The supervision team is completed a non academic supervisor, Prof Filippa Addis, from Porto Conte Ricerche (http://www.portocontericerche.it/). The research will involve secondments to Life Diagnostics (https://lifediagnostics.com/), UK, Porto Conte Ricerche, Italy and MetLabs (http://metlabs.sk/) at Bratislava, Slovak Republic.
The selected candidate will participate in the network’s training activities and work placements in the laboratories of the participating academic and industrial partners.