MANNA - project 09
09it sk

Establishing novel in vitro intestinal epithelial cell models in animal nutrition

Prof. Antonella Baldi
University of Milan
Prof. Mangesh Bhide
University of Veterinary Medicine and Pharmacy in Kosice
University of Glasgow
Prof. Filippa Addis
Porto Conte Ricerche (Italy)
MetLabs (Slovak Republic)

TAGS: in vitro studies; EPS and LPS; intestinal epithelial cells;



The aim of the project is to determine the use of in vitro models of intestinal epithelial cells in order to test the effects of different bioactive and immunomodulatory molecules such as carotenoids and M. cordata extract, PUFA, as well as EPS and LPS.

Description of the project

In the first part of the project the Early Stage Researcher (ESR) will apply cell culture techniques in order to develop models of intestinal epithelia to simulate the in vivo intestinal environment. Two experiments will be carried out. Experiment A: primary lines of intestinal cells will be established, using as starting points the available protocols (target species: pigs and chicken). Experiment B: The lines will be used to elucidate the in vitro impact of molecules used during projects ESR1 (n6/n3 FA in combination with LPS and EPS), ESR3 (antioxidants and M. cordata extract), ESRs 4, 8 and 10 (lipids including CLA), and their effects after in vitro challenge with LPS and EPS. Apoptosis rate, growth rate and the innate immune capability of cells (cytokines and anti-microbial peptides) will be evaluated. Extraction of mRNA and proteins will be carried on for proteomics and transcriptomes analysis.

The ESR will be enrolled by the University of Milan ( under the supervision of Prof. Antonella Baldi and will be awarded a Double Doctorate degree in co-tutelle with the University of Veterinary Medicine and Pharmacy in Kosice (, Slovak Republic, under the supervision of Prof. Mangesh Bhide. The supervision team is completed by Prof Filippa Addis ( at Porto Conte – Italy. The research will involve secondments to University of Glasgow ( UK, and MetLabs ( at Bratislava, in Slovak Republic.

The selected candidate will participate in the network’s training activities and work placements in the laboratories of the participating academic and industrial partners.

Eligibility criteria are indicated at admission rules page

For the specific ESR 9 position, it will be considered advantageous if the candidate has:

  • basic knowledge in cellular biology
  • basic knowledge of laboratory techniques

The University of Milan requires the following additional documents at the moment of enrolment

Proof of evidence for degree titles:

A self-certification might be accepted at the moment of selection, but proofs might be provided at the moment of the enrolment. For the enrolment at the University of Milan they are as follows

  • Master Degree (or equivalent) translated in Italian and authenticated by the authorities of the country where it was obtained
  • Dichiarazione di valore, issued by the Italian Embassy or Consulate in the country where the qualification was obtained
    or, alternatively:
  • Diploma Supplement issued by the University
  • Statement of validity issued by ENIC-NARIC centres (

English language proficiency certificate

The English language proficiency certificate accepted by the University of Milano are listed at


Salary and other benefits for the ESR enrolled at the University of Milan are according to EU-standards for Marie Skłodowska-Curie Actions and national regulations. The living allowance will be €3318.37 per month (gross amount-subject to employers costs, as well as employees tax and insurance) and appointed ESR will be eligible to pay local tax and insurance. Additional benefits are possible in the case of family allowances which will be €500 per month.

Each ESR will also have a Research and Training allowance which will be managed through the University of Milan. This will cover the participation of researchers in training activities;

  • expenses related to research costs;
  • tuition fees (where applicable).
  • Visa, residency card for the recruited researcher and their family (where applicable)

A mobility allowance is also available. This will contribute to mobility related expenses of the ESR. The amount of the mobility allowance is specified in Table 1 of the MSCA Work Programme and for calls 2018-2020 and will amount to €600 per month.

Required documentation

Please refer to the general required documentation at

Application process

The deadline for application is 21st May 2018 through the completion of the form under the section ‘send your application‘.