MANNA - project 09
09it sk

Establishing novel in vitro intestinal epithelial cell models in animal nutrition

University of Veterinary Medicine and Pharmacy in Kosice
http://www.uvlf.sk/en
University of Glasgow
https://www.gla.ac.uk/
MetLabs (Slovak Republic)
http://metlabs.sk/

TAGS: in vitro studies; EPS and LPS; intestinal epithelial cells;

•••••

Objectives

The aim of the project is to determine the use of in vitro models of intestinal epithelial cells in order to test the effects of different bioactive and immunomodulatory molecules such as carotenoids and M. cordata extract, PUFA, as well as EPS and LPS.

Description of the project

In the first part of the project the Early Stage Researcher (ESR) will apply cell culture techniques in order to develop models of intestinal epithelia to simulate the in vivo intestinal environment. Two experiments will be carried out. Experiment A: primary lines of intestinal cells will be established, using as starting points the available protocols (target species: pigs and chicken). Experiment B: The lines will be used to elucidate the in vitro impact of molecules used during projects ESR1 (n6/n3 FA in combination with LPS and EPS), ESR3 (antioxidants and M. cordata extract), ESRs 4, 8 and 10 (lipids including CLA), and their effects after in vitro challenge with LPS and EPS. Apoptosis rate, growth rate and the innate immune capability of cells (cytokines and anti-microbial peptides) will be evaluated. Extraction of mRNA and proteins will be carried on for proteomics and transcriptomes analysis.

The ESR will be enrolled by the University of Milan (http://www.unimi.it/) under the supervision of Prof. Antonella Baldi and will be awarded a Double Doctorate degree in co-tutelle with the University of Veterinary Medicine and Pharmacy in Kosice (http://www.uvlf.sk/en), Slovak Republic, under the supervision of Prof. Mangesh Bhide. The supervision team is completed by Prof Filippa Addis (http://www.portocontericerche.it/) at Porto Conte – Italy. The research will involve secondments to University of Glasgow (https://www.gla.ac.uk/) UK, and MetLabs (http://metlabs.sk/) at Bratislava, in Slovak Republic.

The selected candidate will participate in the network’s training activities and work placements in the laboratories of the participating academic and industrial partners.